Plasmid_Backbone

Part:BBa_K1365301:Design

Designed by: Lisa Hielkema   Group: iGEM14_Groningen   (2014-10-08)


pIL253-mRFP Biobrick Vector for L.lactis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 124
    Illegal suffix found at 1215
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 124
    Illegal NheI site found at 1940
    Illegal SpeI site found at 1216
    Illegal PstI site found at 1230
    Illegal NotI site found at 130
    Illegal NotI site found at 1223
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 124
    Illegal suffix found at 1216
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 124
    Plasmid lacks a suffix.
    Illegal XbaI site found at 139
    Illegal SpeI site found at 1216
    Illegal PstI site found at 1230
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1038
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Cut out the multiple cloning sites of pIL253 also removed illegal restriction sites of EcoRI and XbaI


Source

Yes, Derived from pIL253. Provided by molGen at the university of Groningen.

References